HIV ELISA test kit

HIV ELISA Kit PRINCIPLE The Kinghawk anti-HIV ELISA Kit is a two-step double antigen sandwich ELISA designed to detect antibodies to HIV in human serum or plasma. The test procedure involves three incubation steps: 1. Test serum or sample (properly diluted) is incubated in multi-wells coated with the purified recombinant HIV antigens (gp36 & gp41). Any HIV specific antibodies in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibodies and other serum components. 2. HRP conjugated HIV antigens are added to the wells and the plate is incubated. The Conjugate will react with HIV antibodies immobilized on the solid phase in Step1. The wells are washed to remove unreacted Conjugate. 3. The multi-wells containing immobilized peroxidase conjugate are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time, the reaction is stopped and the resultant photons are counted INTERPRETATION OF RESULTS Cutoff A value=0.10+ N (N = Mean A value of Negative Control. When N is <0.02, it is considered as 0.02) A value of negative must be < 0.10. A value of positive must be >0.50. Otherwise, the assay will be considered false and should be redone. Test Specimen A value < Cutoff A value is considered negative results. Test Specimen A value ≥ Cutoff A value is considered positive results