PUM-T Fast Ligation Kit

I. Component ComponentUltraPower pUM-T Fast Cloning KitDP6701(20 preps)DP6702(60 preps) pUM-T Vector20μl60μl2×Fast Ligation Buffer60μl×2180μl×2T4 DNA Ligase(3 U/μl)20μl60μlControl Insert (500bp)5μl15μl I. Description pUM-T vector is designed for PCR cloning by BioTeke. pUM-T vector derived from pUC18 provides 3’ deoxythymidine (T) by adding certain sequence and specific procedure. Taq polymerase has a non template-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3’ ends of PCR products. This allows PCR inserts to ligate efficiently with the vector. The insert does not change lac Z reading frame, so positive clones can be picked by blue/white screening. In Addition, pUM-T vector contains pUC18 multiple cloning sites. The insert can be confirmed by double digestion or sequenced using M13 universal primers.I. Features Specific rapid ligation buffer ensures ligation in 10-15min.High efficieny and conversion rate, the conversion rate of control segment reach to 95% above.pUM-T contains LacZ for white/blue screening.pUM-T contains pUC18 mutiple cloning sits for confirm