dNTP Mix dATP dGTP dCTP dTTP

Description dNTP Mix is aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. dNTP mix is ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications. The Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. Applications For direct use in PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling. QUALITY CONTROL ASSAY DATA MethodSpecificationResultPurity AssayHPLC column C18; detection UV(at corresponding λmax); mobile phase: A=TEAA 0.1M, pH 7.0; B=60% CH3CN/A) >99%dATP 99.2%dCTP 99.0%dGTP 99.0%dTTP 99.4%LO test (test for detection of exo-, endo-deoxyribonuclease and phosphatase contaminants) Not detectablepassedRibonuclease assay (test for detection of RNase contaminants using [3H]-RNA as a substrate) Not detectablepassedFunctional AssayPCR with Pfu and Taq DNA Polymerases Production of 1000 bp PCR fragment from 2ng of genomic DNApassedpH 7.0-7.5ConcentrationSpectrophotometry 100±5mMdATP 100mMdCTP 100mMdGTP 99mMdTTP 100mM