McAb to CA72-4 (Capture) SPECIFICATION SHEET Catalog #: L1C01101 Lot #: C100401 Description: Monoclonal Antibody to Human CA72-4 Specificity: The CA72-4 is a tumor associated antigen with a molecular weight of >1000Kd. MCF-7carcinoma cells. Clone: B72.3(ML08C01) Host Animal: Mouse. Hybridization of P3-X63-Ag8.653 .myeloma cells with spleen cells from Balb/c mice. Isotype: IgG1. Source: Mouse Ascites Immunogen: BALB/C mice were injected with membrane enriched fraction of a human breast carcinoma liver metastasis. Format: Purified, Liquid Purification: >90% pure (SDS-PAGE). Protein A chromatography. Concentration: 2mg/ml (0.7×OD280nm) Affinity Constant: 5 x 10-9 mol/l. Buffer: 10mM Sodium Phosphate, 0.15M Sodium Chloride, pH 7.4 Preservative: none Applications: Immunohistochemistry on frozen or paraffin sections. Each laboratory should determine an optimum working titer for use in its particular application. Recommended antibody pair for immunoassay: Capture Detection L1C01101 L1C01102 Storage: Upon receipt, aliquot and store at <–40°C. Avoid multiple freeze/thaw cycles. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used.