LSY-10001

Green Spring Nitrofuran (AMOZ) ELISA Test Kit Catalog No. LSY-10001 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This value is compared to the standard curve and the AMOZ concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.05ppb Incubation Temperature: 25℃ Incubation Time: 30min～15min Detection limit Tissue, egg, honey: 0.1ppb Cross-reaction rate AMOZ 100% AHD <0.1% AOZ <0.1% SEM <0.1% Recovery rate Tissue 80±25% Honey 75±25% Egg 95±25% 3.Components 1 Micro-well strips 12 strips with 8 removable wells each 2 6× standard solution (1 mL each) 0ppb 0.05ppb 0.15ppb 0.45ppb 1.35ppb 4.05ppb 3 Enzyme conjugate 7ml red cap 4 Antibody working solution 7ml blue cap 5 Substrate A 7ml white cap 6 Substrate B 7ml black cap 7 Stop solution 7ml yellow cap 8 20× concentrated washing buffer 40ml white cap 9 2× concentrated redissolving solution 50ml transparent cap 10 2-Nitrobenzaldehyde (C7H5NO3) 10ml white cap